Journal: iScience

Article Title: Downregulation of neurodevelopmental gene expression in iPSC-derived cerebral organoids upon infection by human cytomegalovirus

doi: 10.1016/j.isci.2022.104098

Figure Lengend Snippet: NPCs infected with TB40r mGFP-IE-FKBP and administered Shield-1 show positive staining for viral tegument proteins pp65 and pp71 by 24 hpi NPCs were infected at 3 dpi with TB40r mGFP-IE-FKBP at an MOI of 1. One group was then supplemented with 1 μM Shield-1 daily (Shield-1 +) while the other was given vehicle (Shield-1 –), cells were fixed at noted times post infection for staining. (A) Immunocytochemical images from the Shield-1 (−) group at 24, 48, and 72 hpi for viral tegument proteins pp65 or pp71 (purple), neurodevelopmental transcription factor HES1 (red), GFP (green), Hoechst (blue), and merge. (B) Immunohistochemical images from the Shield (+) group using the same staining targets as (A). All images taken at 40X using Zeiss LSM980 with scale bar of 20 uM.

Article Snippet: Rabbit polyclonal anti HES1 , Invitrogen , PA5-28802.

Techniques: Infection, Staining, Immunohistochemical staining

Journal: iScience

Article Title: Downregulation of neurodevelopmental gene expression in iPSC-derived cerebral organoids upon infection by human cytomegalovirus

doi: 10.1016/j.isci.2022.104098

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti HES1 , Invitrogen , PA5-28802.

Techniques: Recombinant, Isolation, SYBR Green Assay, Software

Journal: Neural Regeneration Research

Article Title: Extremely low frequency electromagnetic fields promote cognitive function and hippocampal neurogenesis of rats with cerebral ischemia

doi: 10.4103/1673-5374.301020

Figure Lengend Snippet: Effect of extremely low frequency electromagnetic fields on the expression of Notch1, Hes1 and Hes5 proteins in the subgranular zone. (A) Bands of western blot analysis from the subgranular zone. (B–D) Relative protein expression of Notch1, Hes1 and Hes5. Data are expressed as the mean ± SEM ( n = 6). ## P < 0.01, vs . Sham group; * P < 0.05, vs . Con group (one-way analysis of variance followed by the least significant difference post hoc tests). Con: Control; Exp: experiment; Sham: sham-surgery.

Article Snippet: Equal amounts of proteins were electrophoresed on sodium dodecyl sulfate/10% polyacrylamide gel electrophoresis gels and transferred to nitrocellulose membranes and then incubated with the following primary antibodies: rat anti-Notch1 polyclonal antibody (Cat# 3447; Cell Signaling Technology), mouse anti-β-actin monoclonal antibody (Cat# TA-09; ZSGB-BIO, Beijing, China), rabbit anti-Hes1 polyclonal antibody (Cat# 11988; Cell Signaling Technology) and rabbit anti-Hes5 polyclonal antibody (Cat# ab25374; Abcam).

Techniques: Expressing, Western Blot, Control

Journal: Cell Death Discovery

Article Title: DCLK1 isoforms and aberrant Notch signaling in the regulation of human and murine colitis

doi: 10.1038/s41420-021-00526-9

Figure Lengend Snippet: Representative H&E ( A ), Alcian blue ( B ), and Ki-67 ( C ) staining of uninfected (N), CR-infected (CR), or CR + DBZ-treated (CR + DBZ) Rag1 −/− mice (scale bar 100 μm; 7–8 mice/group; n = 3 independent experiments). D Total crypt RNA from the same group of mice was isolated and Notch1 expression was investigated via real-time RT-qPCR. The values were normalized to GAPDH and data presented as fold change. P values = 0.000004 (Notch1), 0.000091 (Hes1); n = 3 independent experiments. E Western blot analysis of NICD in the isolated crypts in the indicated group. β-Actin was used as the loading control. Lower panel. Densitometry showing relative abundance. * , ** P < 0.05; n = 3 independent experiments. F Real-time RT-qPCR of Notch downstream target Hes-1 in the isolated crypts from the indicated groups. P values = 0.000091 (Hes1); n = 3 independent experiments. Lower panel showing western blot of Hes-1 and Math-1 in the crypts isolated from the indicated groups. Lamin B was used as the loading control ( n = 3 independent experiments).

Article Snippet: Antibodies used were rabbit anti-DCLK1 (1:200, ab31704) and Anti-CR (1:250, ab37056) from Abcam, Cambridge, UK; mouse anti-Notch1 NICD (1:200, clone OTI3E12) from Origene, Rockville, MD; rabbit anti-Hes1 (1:200, #11988) and mouse anti-Ki-67 (1:200, #9449) from Cell Signaling Technology, Danvers, MA; anti-CD68 (1:200, NB600-985) from Novus Biologicals (Littleton, CO, USA), Anti-Muc2 and anti-NE (1:200) Santa Cruz, Dallas, TX; Anti-CD11c and anti-F4/80 (Thermo Fisher), and anti-Ly6G (R&D, Minneapolis, MN).

Techniques: Staining, Infection, Isolation, Expressing, Quantitative RT-PCR, Western Blot